After warming up for the new semester with the yeast meeting, we kicked off our bi-weekly group meetings with the presentation! During summer months we had a pleasure to welcome back project student Sebastian who only a year ago did his bachelor project with us. This time Sebbe was working on a comparison between Non-linear mixed-effects (NLME) and two-stage standard (STS) approaches for analysing single-cell data. Sebastian also developed a dynamic model of Suc2, a downstream target of SNF1. This study showed that both the STS and NLME approaches managed to fit data almost equally good, however, they differed in the ability to simulate the population behaviour, with the NLME approach performing better.